Leica RM2245 User Manual Page 1

Leica RM2245Rotary MicrotomeInstruction ManualLeica RM2245V1.4 English - 02/2007Always keep this manual with the instrument.Read carefully before wor

10Instruction manual V 1.4 – 02/20072.3 Integrated safety devices2. SafetyLocking the handwheelThere are two ways of locking the handwheel (12):Using

11Leica RM22452. SafetyKnife guard on the knife holderEach knife holder is equipped with a tightlymounted knife guard (8, 9). This makes it possi-ble

12Instruction manual V 1.4 – 02/20073.1 Overview – instrument components3. Instrument components and specificationsLeica RM2245Knife guard onthe knife

13Leica RM22453. Instrument components and specificationsFig. 7VentsVoltage selectorswitch with fusesMain switchPowerconnectionConnectingcable forcont

14Instruction manual V 1.4 – 02/20073.3 Technical dataGeneralApprovals: The instrument-specific marks are located next tothe identification label.Nomi

15Leica RM2245MicrotomeSectioning thickness setting:Section thickness setting range: 0.50 - 100 μmSetting values: from 0.50 μm - 5.0 μm in 0.5 μm incr

16Instruction manual V 1.4 – 02/20074.1 Standard deliveryThe Leica RM2245 standard delivery includes:1 Leica RM2245 basic instrument, without orientat

17Leica RM2245Setting up the instrumentDo not transport the instrument by holding it on the handwheelshaft, the object head or the cover!• By holding

18Instruction manual V 1.4 – 02/20074. StartupThe feather key (4) is loosely placed in thehandwheel shaft (1) and fixed in place with atie-rap during

19Leica RM22454. Startup222524 2123The voltage selector is located above the mainpower switch, on the left-rear side of the instru-ment (Fig. 10). The

20Instruction manual V 1.4 – 02/20074. Startup4.5 Switching on the instrumentExposure to extreme temperature changes and high air humidity may cause c

21Leica RM22455. Operation5.1 Operating elements and their functionsThe operating functions of the microtome are divided between a control panel and a

22Instruction manual V 1.4 – 02/20075. OperationThree-digit displayfor section thickness/trimming sectionthicknessLED TRIM (green)Lights up when trim-

23Leica RM22455. Operation5.1.3 Display and control elementsThree-digit displayThis display is located both on the instrumentand on the control panel.

24Instruction manual V 1.4 – 02/2007In sectioning mode the user can select between STEP function (step-by-step specimen feed) and continuous specimen

25Leica RM22455. Operation• To change the display mode, push MENU MODE until the LED of thedesired mode is illuminated.• Press CLEAR to reset section

26Instruction manual V 1.4 – 02/2007Configuring the retraction settings• To call up the retraction settings, press the MENU MODE and CLEARbuttons simu

27Leica RM2245Indication of remaining horizontal feedThe visible and audible remaining feed indica-tion feature informs the user during trimmingand se

28Instruction manual V 1.4 – 02/2007In the quick clamping device of the directional specimen holder fixture, all specimen clampsavailable as optional

29Leica RM2245Fig. 18Fig. 193334Important!Never turn thescrew morethan 1/2turn at a time.5. OperationFine adjustment of the force balanceIf another ac

3Leica RM2245The information, numerical data, notes and val-ue judgments contained in this manual repre-sent the current state of scientific knowledge

30Instruction manual V 1.4 – 02/2007Setting up the knife holder base• Release the clamping lever (50) by rotating itcounterclockwise.• Insert the knif

31Leica RM22455. OperationThere are two versions of the specimen holder,one with and one without specimen orientation,which are interchangeable.The ob

32Instruction manual V 1.4 – 02/20075. Operation• The index marks (0 °, 5 ° and 10 °) for adjust-ment of the clearance angle (59.1) are locatedon the

33Leica RM22455. Operation5.5 Clamping the specimenAlways clamp the specimen block BEFORE clamping the knife.Lock the handwheel and cover the knife ed

34Instruction manual V 1.4 – 02/20075. Operation5.7 Cutting into the specimen (trimming)• Use the TRIM/SECT key to select the trim mode.• Set the desi

35Leica RM22455.9 Changing the specimen or interrupting sectioningLock the handwheel and cover the knife edge with the knife guardprior to any manipul

36Instruction manual V 1.4 – 02/20076.1 Assembly for fixture for specimen clampsDepending upon the purchase order, the basic instrument is delivered w

37Leica RM22456. Optional accessories6.1.3 Fine-directional fixture for specimenclamps• Before the fine-directional fixture forspecimen clamps can be

38Instruction manual V 1.4 – 02/20076.1.4 Quick clamping systemIt is used as specimen holder for use with thefine-directional fixture for specimen cla

39Leica RM22456. Optional accessories6.2.1 Standard specimen clampThe standard specimen clamps are available intwo sizes: 40 x 40 mm and 50 x 55 mm.Th

4Instruction manual V 1.4 – 02/2007TABLE OF CONTENTS1. Important notes ...

40Instruction manual V 1.4 – 02/20076. Optional accessoriesClamping of foil pieces• Move the movable jaw (74) to the right asrequired by turning the s

41Leica RM2245• To open the jaws (78) and (79) lightly loosenthe 3 screws (81) with an Allen key size 4(71).• Insert the foil ribbon (72) from behind

42Instruction manual V 1.4 – 02/200765The universal cassette clamps (UCC) aredesigned for horizontal or verticalclamping of all kinds of commercialcas

43Leica RM2245Assembly of the super mega-cassette clampThe super mega-cassette clamp should preferably be used with the rigidfixture for specimen clam

44Instruction manual V 1.4 – 02/2007• To mount the required insert (89.1-3) turn theclamping ring (90) counterclockwise andremove it.• Place the requi

45Leica RM22456. Optional accessories6.3 Knife holder base and knife holderRepositioning the knife holder baseThe one-piece knife holder base (rigid)

46Instruction manual V 1.4 – 02/20076. Optional accessoriesPrior to inserting the blade, both knifeholder and knife holder base must havebeen installe

47Leica RM22456. Optional accessoriesLateral displacement (only for knife holder E)The lateral movement feature of the knifeholder base enables the us

48Instruction manual V 1.4 – 02/2007Knife holder E with water bath for narrow-band and broad-band blades6. Optional accessoriesFig. 42Knife holder E w

49Leica RM22456.3.3 Knife holder N/NZThe knife holders N and NZ are appropriate for standard steel and tungsten carbide knives, profilec and d, up to

5Leica RM2245TABLE OF CONTENTS6.1.3 Fine-directional fixture for specimen clamps ...

50Instruction manual V 1.4 – 02/20076. Optional accessoriesInserting the knife• Rotate the knurled nuts (48) on the right and left of the knife holder

51Leica RM22456. Optional accessoriesThe backlighting is inserted at the frontinto the one-piece knife holder base.• Remove the two screws (1) using a

52Instruction manual V 1.4 – 02/20076. Optional accessories6.7 Freezer packFig. 48989998Fig. 476.6 TrayThe freezer pack consists of the freezer plate(

53Leica RM22456.8 Universal microscope carrier6. Optional accessories1 - Baseplate with drilling (a)2 - Vertical column with size 8 cap screw (b) and

54Instruction manual V 1.4 – 02/20072. Attach vertical column• Insert the cap screw (b) into the hole of thebaseplate from below. Place the lock washe

55Leica RM22456. Optional accessories6.9 Magnifying lensFig. 55Fig. 57Fig. 5613The magnifying lens provides a 2xmagnification and can be used with all

56Instruction manual V 1.4 – 02/200724566. Optional accessories6.9 Cold light source6.10 Fiber-optic light guideFig. 62, Leica rotary microtome with i

57Leica RM22456.11 Ordering informationFixture for specimen clamps, rigid, silver ...

58Instruction manual V 1.4 – 02/20076. Optional accessoriesEM specimen holder fixture, black ...

59Leica RM22457. Customized solutionsCertain instrument malfunctions appear on the 3-digit LED displayor are indicated through illuminated LED’s on th

6Instruction manual V 1.4 – 02/2007Function keys which must be pressed onthe control panel are shown in the textin bold capital letters.1.1 Symbols in

60Instruction manual V 1.4 – 02/20077. Customized solutions• Feed motion no longer occurs.Problem Corrective actionPossible cause1. STEP mode is still

61Leica RM22457. Customized solutions1. Thick/thin sectionsThe sections alternate betweenbeing thick and thin. In extremecases, there are no sectionsw

62Instruction manual V 1.4 – 02/2007Cleaning the knifeAlways wipe the knife from the back of the knife to the cutting edge. NEVER wipe in theopposite

63Leica RM22458. Cleaning and maintenanceKnife holder EFig. 6110119Fig. 6286838386871110Take the knife holder apart for cleaning. To doso, proceed as

64Instruction manual V 1.4 – 02/2007Universal cassette clamp• Detach cassette clamp (13) for a thorough cleaning, removing allparaffin residues.• For

65Leica RM22458.2.2 Maintenance instructionsOnly authorized and qualified service personnel may access the internal components ofthe instrument for se

66Instruction manual V 1.4 – 02/20078. Cleaning and maintenance978873111090Universal cassette clamp:• Shaft (90) and flat coil spring (91) of theclamp

67Leica RM22459. Warranty and serviceWarrantyLeica Microsystems Nussloch GmbH guarantees that the contractualproduct delivered has been subjected to a

. . . . . . . . . . . . . . . . . . . . . . . . . . . .Dag GraupnerManaging DirectorEC Declaration of ConformityLeica Microsystems Nussloch GmbHPostfa

7Leica RM22452. Safety2.1 Safety notesThis instruction manual contains importantinstructions and information regarding theoperational safety and maint

8Instruction manual V 1.4 – 02/20072. SafetyWarnings – Safety instructions / warning labels attached to the instrument• Safety instruction labels on t

9Leica RM2245Warnings – Working at the instrument• Take care when handling microtome knives and disposable blades. The cutting edge isextremely sharp