Leica M205 User Manual

Leica M165 FCLeica M205 FAUser Manual

Leica M-series User Manual 10Quick Start GuideThe Fastest Route to Success 64Overview of an M Series Microscope 65The Correct Interpupillary Dista

Leica M-series User Manual 100Leica TL RCI™: Relief ImagesStarting position1. Push the deflection mirror all the way back towards the column.2. Tur

Leica M-series User Manual 101Dynamic relief contrastSuitable for semitransparent and transparent specimens. By tilting the deflection mirror sli

Leica M-series User Manual 102Filters for Leica TL RC™ and TL RCI™The transmitted-light bases TL RC™ and TL RCI™ can be equipped with up to th

Leica M-series User Manual 103Leica IsoPro™ (Non-motorized): ControlsOperating the Leica IsoPro™ mechanical stage 1. To move the stage in the X dir

Leica M-series User Manual 104Leica IsoPro™ (Motorized): Controls1 Leica IsoPro™ motorized mechanical stage2 Housing with motorization1 Sub-D int

Leica M-series User Manual 105System Illumination

Leica M-series User Manual 106UseThe Leica LED5000 MCI™ (for "Multi Contrast Illumination") is a universal high-output illu-min

Leica M-series User Manual 107Using the keyboardUse the ρ key to switch the illumination on or off. Use the "+" and "–" key

Leica M-series User Manual 108Leica LED5000 RLUseThe Leica LED5000 RL ("Ring Light") generates a very bright and homogenous incident

Leica M-series User Manual 109The Leica EL6000 compact light source is intended for biological routine and research work, including the e

Leica M-series User Manual 11System IlluminationLeica LED5000 MCI™ 106Leica LED5000 RL 108Leica EL6000 – About the Instrument 109Leica EL 6000 –

Leica M-series User Manual 110Leica EL6000 – About the Instrument (cont'd.)Compatible light guidesThe Leica EL6000 compact light source i

Leica M-series User Manual 111Leica EL 6000 – OperationControls Running-time meterThe running-time meter measures the burn-ing time of the

Leica M-series User Manual 112Leica EL 6000 – TroubleshootingFault description Possible faults RemedyPower indicator is not illuminated after being

Leica M-series User Manual 113Cleaning and Maintenance•Before carrying out cleaning tasks, switch off the Leica EL6000 compact light source

Leica M-series User Manual 114Fluorescence

Leica M-series User Manual 115Filter ChangerThe patented FLUOIII® filter system consists of a filter changer for barrier and excitation filters, a UV s

Leica M-series User Manual 116FIM – Fluorescence Intensity ManagerFIM consists of a diaphragm disk with pinhole diaphragms of varying transmissio

Leica M-series User Manual 117About Fluorescence MicroscopyFunctional principleFluorescent substances light up when irradi-ated with short-wave

Leica M-series User Manual 118Rapid Filter Changers and Filter TypesFilter sets Excitation filter Barrier filter DesignationGFP 425/60 nm (395–455 nm)

Leica M-series User Manual 119Simple filter holdersSimple filter holders Three simple filter holders are included with the Leica M165 FC and the

Leica M-series User Manual 12The Leica M Series for Fluorescence

Leica M-series User Manual 120Equipping the Filter Changer•When inserting the filter sets, avoid touching the filters in order to avoid fin

Leica M-series User Manual 121Observation without FluorescenceWe recommend that you first familiarize yourself with the controls of your Leica

Leica M-series User Manual 122Double Iris ApertureThe built-in double-iris diaphragm regulates the depth of field. Note that:The greater the d

Leica M-series User Manual 123Commissioning the Fluorescence SystemAfter switching on the high-pressure mercury burner, you must adjust the

Leica M-series User Manual 124Accessories

Leica M-series User Manual 125With its non-slip controllers and knobs, the Leica SmartMove control unit offers the right feel for handlin

Leica M-series User Manual 126With the touchscreen of the Leica SmartTouch™, you can control your experiment procedures with a few hand mov

Leica M-series User Manual 127Leica PSC ControllerUseThe Leica PSC controller gives you precision control of the Leica IsoPro™ motorized mecha

Leica M-series User Manual 128Dimensional Drawings

Leica M-series User Manual 129Leica M165 FCLeica M165 FC with incident light base and new focusing column (dimensions in mm)385154340 3904409515

Leica M-series User Manual 13Congratulations!Congratulations on obtaining your new Leica M series stereomicroscope. We are convinced it will exceed

Leica M-series User Manual 130Leica M165 FC (cont'd.)Leica M165 C with TL RCI™ transmitted light stand, Leica IsoPro™ manual mechanical stage a

Leica M-series User Manual 131Leica M165 FC (cont'd.)Leica M165 C with TL RCI™ transmitted light stand and manual focus340 4308515435615429

Leica M-series User Manual 132Leica M205 FALeica M205 FA with TL BFDF transmitted light stand, ErgoTrinoc tube, DFC camera and motorized focusing dr

Leica M-series User Manual 133Leica M205 FA (cont'd.)Leica M205 FA with TL RCI™ transmitted light stand, ErgoTrinoc tube, DFC camera and motori

Leica M-series User Manual 134Leica M205 FA (cont'd.)Dimensions of Leica M205 FA with TL RCI™ transmitted light stand, IsoPro™ mechanical stage

Leica M-series User Manual 135Leica LED5000 MCI132275.315783.511245112904536 22132275.315783.511245112904536 22132275.315783.511245112904536 22Leica

Leica M-series User Manual 136Leica LED5000 RL120ø7030153153ø80

Leica M-series User Manual 137MATS TL1901402204111349817010 447 16410 447 275

Leica M-series User Manual 138Specifications

Leica M-series User Manual 139Leica M165 FC / Leica M205 FALeica M165 FCLeica M205 FAOptical dataZoom16.5 : 1 manual20.5 : 1 automated with FusionOp

Leica M-series User Manual 14A Step Towards InfinityEver since their introduction by Horatio S. Greenough, stereomicroscopes have worked acc

Leica M-series User Manual 140Leica M165 FC / Leica M205 FA (cont’d.)Leica M165 FCLeica M205 FAOptics carrier100% apochromatic optical systemCMO (Co

Leica M-series User Manual 141Leica M165 FC / Leica M205 FA (cont’d.)Leica M165 FCLeica M205 FAAdditional objectives (non-parfocal)1× planachromat,

Leica M-series User Manual 142Leica M165 FC / Leica M205 FA (cont’d.)Leica M165 FCLeica M205 FAIncident light standLarge incident light base with bl

Leica M-series User Manual 143Leica EL6000 – SpecificationsDimensions (W×H×D)Width×height×depth 130 mm × 210 mm × 290 mmEnvironmental conditionsPermi

Leica M-series User Manual 144Leica EL6000 – Specifications (cont'd.)Light outputDesign Type: Storz, longSpecial feature Self-locking if no ligh

Leica M-series User Manual 145Leica TL ST Transmitted-light BaseLight source Halogen lamp 12V/20WQuick illuminant change YesIlluminated area 50 mmPo

Leica M-series User Manual 146Leica TL BFDF Transmitted-light BaseLight source External via cold light sourceIlluminated area 40 mmConnections Conne

Leica M-series User Manual 147Leica TL RC™ / TL RCI™Leica TL RC™ Leica TL RCI™Light source External via cold light source Halogen lamp 12V/20WQuick

Leica M-series User Manual 148Leica IsoPro™ Motorized XY StageCompatibility Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™) Stage

Leica M-series User Manual 149Appendix

Leica M-series User Manual 15The Electronics: Comfort, Convenience and Safety for Your ExperimentsNever before have electronics been used as e

Leica M-series User Manual 150Calculating the Total Magnification and Field of View DiameterParameterMO Magnification of the objective ME Magnification

Leica M-series User Manual 151Care, Maintenance, Contact PersonsWe hope you enjoy using your high-perfor-mance microscope. Leica microscope

Leica M-series User Manual 152Care, Maintenance, Contact Persons (cont'd.)Protection from dirtDust and dirt will affect the quality of y

Leica M-series User Manual 16Have a special request? Let us know!Leica Microsystems enjoys an exceptional repu-tation when it comes to devising

Leica M-series User Manual 17Leica engineers were careful to ensure that the new Leica M series—like its predecessors—remains compatible with

Leica M-series User Manual 18On We GoIf your new Leica microscope has already been assembled and commissioned by your Leica consultant, click h

Leica M-series User Manual 19Assembly

Leica M-series User Manual 2General InstructionsSafety conceptBefore using your microscope for the first time, please read the "Safety conce

Leica M-series User Manual 20The first step is to connect the focusing column of the M series to the corresponding base.Tools usedHex socket screwdri

Leica M-series User Manual 21Assembling the Focusing Column with an Incident-light BaseWhen using an incident-light base, the focus-ing colum

Leica M-series User Manual 22Depending on the work situation, it is useful to restrict the maximum travel path of the stereomicroscop

Leica M-series User Manual 23Tools usedHex socket screwdriver, 4 mm ρAssembling the optics carrier1. Place the optics carrier on the focusing

Leica M-series User Manual 24TubeAll intermediate tubes that fit between the optics carrier and the binocular tube are fitted in the same manner

Leica M-series User Manual 25EyepiecesTools usedNo tools required. ρMagnification rangeYou can extend the overall magnification range using available

Leica M-series User Manual 26ObjectiveTools usedNo tools required. ρPreparation1. Remove the protective cap on the optics carrier by turning

Leica M-series User Manual 27UV Protection Screen: AssemblyTools usedAllen key ρIntended useThe UV protection screen in front of the speci-men plan

Leica M-series User Manual 28Preparations•Hold the objectives firmly during assembly and disassembly so that they do not fall onto the stage pla

Leica M-series User Manual 29Objective Nosepiece – Adjusting ParfocalityThe following procedure only has to be carried out once. Afterwards, both ob

Leica M-series User Manual 3Important Safety NotesUser ManualThe individual modules of the Leica M stereo-microscopy series include an interact

Leica M-series User Manual 30Transmitted-light Base TL STUnpacking the baseThe base is delivered with the adapter plate installed. Make sur

Leica M-series User Manual 31FluoCombi III™ – PreparationsBefore assembling the FluoCombi™, you have to disconnect all instruments from the powe

Leica M-series User Manual 32FluoCombi III™ – Assembly1. Guide the FluoCombi III™ in from below, while holding it flat. Ensure that it is alig

Leica M-series User Manual 33Assembling the optics carrier1. Unscrew the three screws and the adapter ring on the optics carrier.2. Attach the opt

Leica M-series User Manual 34FluoCombi III™ – Adjusting the ParfocalitySetting up the objective nosepieceThe following configuration only has to be

Leica M-series User Manual 35FluoCombi III™ – Adjusting the Parfocality (cont'd.)9. By turning the objective ring, focus on the specimen until

Leica M-series User Manual 364. Center the objective over the specimen by loosening or tightening the screw on one side of the objective hol

Leica M-series User Manual 37FluoCombi III™ – Filter and UV Protection ScreenInserting the dichroic mirror1. Pull the dummy filter carrier out

Leica M-series User Manual 38Removing the transport anchors•Before you can use the transmitted-light base for the first time, it is abso-

Leica M-series User Manual 39Transmitted-light Base TL BFDFStandard deliveryThe base is delivered with the adapter plate installed. The sel

Leica M-series User Manual 4Symbols UsedWarning of a danger•This symbol indicates especially impor-tant information that must be read and complied

Leica M-series User Manual 40The base is delivered with the adapter plate installed. The selected stage (Leica IsoPro™ mechanical stage

Leica M-series User Manual 41IsoPro™ Manual Mechanical Stage: AssemblyBefore the Leica IsoPro™ mechanical stage is mounted to the base, th

Leica M-series User Manual 42Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.)1. Take the glass plate from the mechanical stage

Leica M-series User Manual 43Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.)3. Attach the mechanical stage evenly to the three threa

Leica M-series User Manual 44BasicsThe transmitted light bases of the Leica TL series (TL BFDF, TL RC™, TL RCI™) are supplied with an installed ext

Leica M-series User Manual 453. Remove the two M3 screws and the holder from the mechanical stage.4. Remove the two M4 screws and the sleeve from

Leica M-series User Manual 46Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)The basesThe TL BFDF, TL RC™ and RCI™ transmitted-ligh

Leica M-series User Manual 47Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)Mechanical stage for X-Y stage control module1. Plug

Leica M-series User Manual 48The new Leica M-series features extensive encoding with which various microscope data and settings can be read ou

Leica M-series User Manual 49The integrated cable duct in the column enables a neat cable layout around the microscope. For example, the USB o

Leica M-series User Manual 5Safety InstructionsDescriptionThe individual modules fulfill the highest requirements for observation and documenta-

Leica M-series User Manual 50Cables: DiagramTL RCI™DCI ControlboxPSC SmartTouchPC USBCross StageSmart MovePower/USBLED5000 RLLED5000 MCI™Footswitch(

Leica M-series User Manual 51The Leica LED5000 MCI™ (for "Multi Contrast Illumination") is installed using two screws. For optimum a

Leica M-series User Manual 52Under certain circumstances, the light source must not be moved along with the optics carrier. A typical ex

Leica M-series User Manual 53The ring illuminator, the Leica LED5000 RL ("Ring Light"), is installed on the objective using a single

Leica M-series User Manual 54•The manufacturer assumes no respon-sibility or liability for any use outside of the intended use or use outside of

Leica M-series User Manual 55Leica EL6000 – Safety Notes (cont'd.)•The Leica EL6000 compact light source generates high-energy light with invi

Leica M-series User Manual 56Leica EL6000 – Safety Notes (cont'd.)Electrical safetyFor indoor use only.Supply voltage 100–240 V~ (±10%)Frequenc

Leica M-series User Manual 57Setting up the instrument•Set up the Leica EL6000 so that the front is readily accessible and visible.The ventilation

Leica M-series User Manual 58Leica EL6000 – Preparation (cont'd.)4. Carefully lay the Leica EL6000 on its side so that the opening faces you.T

Leica M-series User Manual 59Leica EL6000 – Connection to the Fluorescence Lamp Housing•Always connect the light guide to the lamp housing o

Leica M-series User Manual 6Safety Instructions (cont'd.)Repairs, service workRefer to "Safety Concept" booklet ▶Only original Leic

Leica M-series User Manual 60Leica EL6000 – Preparation (cont'd.)7. When the lamp makes full contact with the lamp mount, release the lever.8.

Leica M-series User Manual 61Leica EL6000 – Replacing the LampThe procedure for replacing the lamp does not differ from the installation procedure ou

Leica M-series User Manual 62Leica EL6000 – Connection to the Fluorescence Lamp Housing (cont'd.)6. Insert the long end of the light guide int

Leica M-series User Manual 63Quick Start Guide

Leica M-series User Manual 64The Fastest Route to SuccessYour Leica stereomicroscope has been delivered in completely assembled condition by your

Leica M-series User Manual 65Overview of an M Series Microscope1 Eyepieces for spectacle wearers with dioptric correction and eyecups2 Trinocular

Leica M-series User Manual 66The Correct Interpupillary DistanceThe interpupillary distance is correctly set if you see a single circular image fi

Leica M-series User Manual 67If you wear glasses:1. Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear, as otherw

Leica M-series User Manual 68FocusingFocusing raises or lowers the stereomicroscope using the focusing drive. The specimen detail is brought into sh

Leica M-series User Manual 69Adjusting the Resistance of the Focus DriveAdjusting the resistanceIs the focus movement too loose or too tight? Does

Leica M-series User Manual 7Safety Instructions (cont'd.)•Direct contact with eyepieces is a poten-tial transmission method for bacterial

Leica M-series User Manual 70Changing Magnification (Zoom)All M series microscopes have an integrated zoom. The name indicates the zoom r

Leica M-series User Manual 71The zoom button can optionally be operated either with or without ratchet steps. Continu-ous zoom is possibl

Leica M-series User Manual 72Parfocality: More Comfort and Convenience for Your WorkAll Leica stereomicroscopes are parfocally matched, meaning

Leica M-series User Manual 73The iris diaphragm in the optics carrier of your M series microscope fulfills the same purpose as the iris diaphr

Leica M-series User Manual 74Eyepieces

Leica M-series User Manual 75Magnification Factors of the EyepiecesAn eyepiece not only makes it possible to look passively into the micro-scope, but

Leica M-series User Manual 76Health NotesPotential sources of infection•Direct contact with eyepieces is a potential transmission method fo

Leica M-series User Manual 77All Leica eyepieces are also available with built-in dioptric correction, allowing the microscope to be used without gl

Leica M-series User Manual 78Adjusting1. Set the dioptric correction for both eyepieces to "0".2. Select the lowest magnification a

Leica M-series User Manual 79Graticules2. Clamp the graticule on the insert, applying moderate pressure. Ensure that the grati-cule fits tightl

Leica M-series User Manual 8Safety Instructions (cont'd.)Supply unitAlways unplug the supply unit from the power supply:When installing and

Leica M-series User Manual 80Photography & Video

Leica M-series User Manual 81For most microscope users, digital documen-tation has become an invaluable part of their work. Research res

Leica M-series User Manual 82Photo Tubes and C-mountsApplicationAll Leica DFC cameras are equipped with a standardized C-mount interface.

Leica M-series User Manual 83Trinocular Video/Phototube 50%UseWith its third beam path, the trinocular video/phototube 50% enables you to simultaneo

Leica M-series User Manual 84UseWith its third beam path, the trinocular video/phototube 100% enables you to either view or photograph a specimen. T

Leica M-series User Manual 85Microscope Carrier

Leica M-series User Manual 86UseThe objective nosepiece enables you to switch between two objectives using just one hand movement, for example

Leica M-series User Manual 87Objectives and Optical Accessories

Leica M-series User Manual 88The Different Types of ObjectivesTo meet the various requirements regarding imaging properties, there is a cho

Leica M-series User Manual 89Bases

Leica M-series User Manual 9ContentsGeneral Instructions 2Important Safety Notes 3Symbols Used 4Safety Instructions 5The Leica M Series for Fluo

Leica M-series User Manual 90Leica TL ST Transmitted-light Base: Controls1 Adapter plate for easy assembly of focusing drives2 Removable glass pla

Leica M-series User Manual 91Leica TL ST Transmitted-light Base: OperationLight intensity controlThe left control adjusts the intensity of th

Leica M-series User Manual 92Changing the halogen lampBefore you change the lamp, it is abso-lutely necessary to unplug the power plug from th

Leica M-series User Manual 93Leica TL BFDF Transmitted-light Base: Controls1 Adapter plate for easy assembly of focusing drives2 Standard stage 10

Leica M-series User Manual 94Light intensity control•Observe the user manual—in particu-lar, all safety regulations—from the manufacturers

Leica M-series User Manual 95Leica TL RC™ / TL RCI™: ControlsVertical column - transmitted-light base adapter plate1 Heat sink of the integrated h

Leica M-series User Manual 96Leica TL RCI™: The Deflection MirrorInverted world?Depending on the properties of the specimen (refractive index of

Leica M-series User Manual 97Leica TL RCI™: Color Intensity and Temperature1 Potentiometer for controlling the CCIC™ (Constant Color Intensity Cont

Leica M-series User Manual 98Setting the relief contrastThe two switches on the left side of the trans-mitted-light base TL RC™/TL RCI™ activate two

Leica M-series User Manual 99Leica TL RCI™: Methods in Transmitted LightVertical bright-field illumination Suitable for stained amplitude specimens w