Leica CM1950 User Manual

Operating ManualLeica CM1950, V 1.1 English – 05/2007Always keep this manual near the instrument.Read carefully prior to working with the instrument!L

10Operating Manual V 1.1 – 05/2007To prevent adverse health effects from UV radiation, the UV disinfec-tion cycle can be started only after the slidin

11Leica CM1950 – Cryostat2.4 Securing/Locking the HandwheelAlways lock the handwheel and cover the cutting edge with theknife guard prior to manipula

12Operating Manual V 1.1 – 05/20072. Safety and Design• When disinfecting the instrument, take appropriate protective measures(gloves, mask, protectiv

13Leica CM1950 Cryostat2. Safety and Design2.7 Removal of the Microtome• The microtome is encapsulated and therefore does not require removalby the us

14Operating Manual V 1.1 – 05/20073. Technical Data② according to IEC-1010; UL 3101TypeNominal voltage (±10 %)Nominal frequencyPower drawMax. start-up

15Leica CM1950 – Cryostat3. Technical DataRefrigeration system CM1950, 50 Hz CM1950, 60 HzCryochamberTemperature range 0°C to -35°C ± 5 K, adjustable

16Operating Manual V 1.1 – 05/20074. Standard DeliveryA choice of different knife holders is available for the CM1950.Compare the delivered components

17Leica CM1950 – Cryostat4. Standard DeliveryBasic instrument WITH motor/WITHOUT extraction, in the specific voltage variant1 handwheel, motorized ..

18Operating Manual V 1.1 – 05/20075. General OverviewCastors for safe transportover short distancesHandwheel in12 o’clockpositionOn/off switch, also c

19Leica CM1950 – Cryostat5. General Overview5.1 Control Panel Fields and Cryostat Chamber1231 - Control panel field 1: Extraction, temperature and ti

20Operating Manual V 1.1 – 05/20076. Installation6.1 Site RequirementsThe place of installation must meet the following requirements:• No direct sunli

21Leica CM1950 – Cryostat6. Installation6.2 Transport to the Site• First, check if the location meets the conditions specified in "Siterequireme

22Operating Manual V 1.1 – 05/2007• Insert the pin (1) of the handwheel shaft intothe hole (2) of the handwheel.• Tighten the screw (4) using the No.

23Leica CM1950 Cryostat6.3.1 Locking/Unlocking the HandwheelOnly rotate the handwheel when the refrigeration system is on andthe cryochamber is cold!A

24Operating Manual V 1.1 – 05/20076.4 Connection to Power Supply SystemAfter transporting, wait at least 4 hours before turning the instrument on. Thi

25Leica CM1950 – Cryostat6.5 Installing Accessories/Inserting Chamber Accessories6.5.1 Installing the Adjustable Footrest (Optional)• To install the

26Operating Manual V 1.1 – 05/20076.5.2 Installing the Storage Systems (Optional)6.5.3 Shelf, Movable (Optional)Attach the rod for the shelf to the i

27Leica CM1950 – Cryostat6. Installation6.5.5 Installing the Heat Conductor Block, Stationary (Optional)Fig. 246.5.6 Installing the Knife/Blade Holde

28Operating Manual V 1.1 – 05/2007Reasons:1. To prevent section waste from falling into theopening.2. To prevent cold from escaping from thechamber.3.

29Leica CM1950 – Cryostat6.5.9 Installing the Section Extraction (Optional) – Use with Blade Holder CE Only6. InstallationIf the extraction is not us

3Leica CM1950 – CryostatPublished by:Leica Biosystems Nussloch GmbHHeidelberger Str. 17 - 19D-69226 NusslochGermanyPhone: +49 (62 24) 143-0Fax: +49 (

30Operating Manual V 1.1 – 05/20077. Instrument ControlsDisplay of actual and target specimenhead temperaturesDisplay of actual and target chambertemp

31Leica CM1950 Cryostat• The Peltier element provides additional cooling for the freezing stations.After the button is pressed, the display ch

32Operating Manual V 1.1 – 05/2007For trimming values above a sec-tion thickness of 200 μm, the dis-play flashes to expressly notifythe user of thick

33Leica CM1950 – Cryostat7. Instrument ControlsCoarse feed functionsThe two-speed electric coarse feed is designed to quickly position the spec-imen

34Operating Manual V 1.1 – 05/2007Sectioning modesThe instrument can be used in manual and motorized modes.The following settings are available:• Sing

35Leica CM1950 – Cryostat7. Instrument ControlsDisinfectionDuration - 30minDuration - 180 minUVC key - to activate / deac-tivate the disinfection cyc

36Operating Manual V 1.1 – 05/20078. Working with the Instrument8.1 Preparing Cutting Tools, Specimen Discs and Preparation AidsThe knives are extreme

37Leica CM1950 Cryostat8. Working with the Instrument8.3 Configuring the ParametersThe instrument must be switched on at least 5 hours before theplann

38Operating Manual V 1.1 – 05/2007Setting the time• The clock time is set with the +/- buttons in the control panel markedwith the clock symbol.To do

39Leica CM1950 Cryostat8. Working with the InstrumentProgramming the defrost cycle• Set the beginning of the automatic defrost cycle.The automatic def

4Operating Manual V 1.1 – 05/2007Table of contents1. Important Information...

40Operating Manual V 1.1 – 05/20078. Working with the InstrumentManual defrosting of the cryochamber• Push the manual defrost button (with the melting

41Leica CM1950 Cryostat8. Working with the InstrumentEntering the trimming thicknessTo switch from a trimming section thickness for research applica-t

42Operating Manual V 1.1 – 05/20078. Working with the Instrument8.4 Working with the Precooled Cryostat8.4.1 Preparatory Work• Lock the handwheel in t

43Leica CM1950 – Cryostat8. Working with the Instrument8.4.2 Trimming with Extraction – Anti-roll Guide Installed• Remove the silicone stopper from t

44Operating Manual V 1.1 – 05/20078. Working with the InstrumentFor motorized sectioning, always begin at low speed for safetyreasons.• Optimize the v

45Leica CM1950 Cryostat8. Working with the Instrument• Activate trimming mode.• Select the trimming thickness.• Switch on the VAC and select a low ext

46Operating Manual V 1.1 – 05/2007A few rules:• Always begin at a low extraction level, then slowly increase it.• Do not use high extraction levels un

47Leica CM1950 Cryostat8. Working with the InstrumentAfter trimming or sectioning:Specimen:• Unclamp and thaw.• Immerse in fixative for further proces

48Operating Manual V1.1 – 05/2007Problem Causes RemediesFrost on chamber walls andmicrotomeSections smearSections splinterSections not properlyflatten

Leica CM1950 Cryostat49Problem Causes RemediesRidged sectionsChatter during sectioningCondensation on anti-rollplate and knife duringcleaningAnti-roll

5Leica CM1950 – Cryostat7. Instrument Controls...

50Operating Manual V1.1 – 05/2007Problem Causes RemediesBlunt knife.Inappropriate section thickness.Incorrect clearance angle.Microtome not dried thor

Leica CM1950 Cryostat51Problem Causes RemediesNo or insufficientrefrigeration of thecryochamberSliding window collectscondensationNo or insufficientre

52Operating Manual V 1.1 – 05/200710. Temperature Selection Chart (in minus °C)Tissue type Chamber temperature Specimen head temperatureSpleen -15°C t

53Leica CM1950 – Cryostat11.1 Ordering InformationSpecimen disc ø 20mm assembly ... 0477 43739Specim

54Operating Manual V 1.1 – 05/2007Section waste tray ... 0477 40062Brush sh

55Leica CM1950 – CryostatAssembling the anti-roll guide system(for the blade holder CE)1. Insert the glare-minimizing glass insert into theinterchang

56Operating Manual V 1.1 – 05/2007The knife guard is foldedaway in the detail view.Knife guard folded awayFig. 43Four possiblesettingsBlade rest (rule

57Leica CM1950 – CryostatAnti-roll guide system functions as aknife guard here!1. Fold the anti-roll guide system (4) to the left –while doing so, ho

58Operating Manual V 1.1 – 05/200711. Optional AccessoriesAdjusting the Knife Holder with Anti-roll GuideYou can adjust the height of the anti-roll gu

59Leica CM1950 – CryostatCleaning the knife holder CNDaily cleaningFor daily cleaning, it is enough to fold theanti-roll guide system forwards and re

6Operating Manual V 1.1 – 05/20071. Important Information1.1 Symbols Used in This Manual andTheir MeaningNotesi.e. important user information appearin

60Operating Manual V 1.1 – 05/20071Knife holder CN with anti-roll guide – Movingthe clamping chucksThe large specimen disc (80x 50 mm) should pref-era

61Leica CM1950 – CryostatKnife holder CNZ with anti-roll guide• Pressure plate permits the full length of theknife to be utilized.• Use hard metal an

62Operating Manual V 1.1 – 05/2007Fig. 56Bacterial filter• Bactericidal filter 350/5865, pack of 1 unit. Rec-ommendation: replace bactericidal filter

63Leica CM1950 – CryostatFig. 57Fig. 58Replacement filters for extraction system(pack of 25)• Change when vacuum power is impaired.• When using the c

64Operating Manual V 1.1 – 05/2007Fig. 60A Heat extractor with parking station, stationary• for installation in the cryostat - consisting of:support d

65Leica CM1950 – CryostatFootswitch,for use with motorized instruments onlyFig. 63Fig. 66Fig. 6511. Optional AccessoriesBrush shelf,for use with blad

66Operating Manual V 1.1 – 05/200712. Maintenance and Cleaning1Fig. 6712.1 General MaintenanceThe microtome is virtually maintenance-free. To ensur

67Leica CM1950 Cryostat12. Maintenance and CleaningBasically, we recommend UV disinfection (see page 35 for detailedinstructions).For easy-to-use spra

68Operating Manual V 1.1 – 05/200712.3 Replacement of the Lamp/UVC LampTurn the instrument off with the automatic power fuse and pull thepower plug, b

69Leica CM1950 – CryostatInstallation of the new lampType:OSRAM DULUX L 18W/ 840.• Hold the lamp in the correct mounting posi-tion as shown and push

7Leica CM1950 – Cryostat2.1 General Safety InstructionsThe CM1950 is a cryostat with an encapsulated microtome and separatespecimen cooling. It is pr

70Operating Manual V 1.1 – 05/200713. Decontamination Certificate (master)Dear Customer,Any product which is to be returned to Leica Microsystems or s

71Leica CM1950 – Cryostat13. Decontamination Certificate (master)Leica Biosystems Nussloch GmbHHeidelberger Str. 17-1969226 Nussloch, GermanyPhone: +

We herewith declare, in exclusive responsibility, that theLeica CM1950 Cryostatwas developed, designed and manufactured to conform with the• Council

73Leica CM1950 Cryostat15. Warranty and ServiceWarrantyLeica Biosystems Nussloch GmbH guarantees that the contractual productdelivered has been subjec

74Operating Manual V 1.1 – 05/200716. People's Republic of China

8Operating Manual V 1.1 – 05/20072. Safety and DesignOpening the packaging1. Remove the 3 screws (1) on each of the two sides of thebox (7) at the top

9Leica CM1950 Cryostat2. Safety and DesignRemoving the packaging1. Remove the cover (17) and place it on the floor with the foam parts facingdownwards